Hydrogen bonding in between Tyr 641 and the substrate lysine’s -nitrogen, and steric envelope of the tyrosine hydroxy group impose rotational constraints that penalize proper alignment with the cofactor’s scissile bond, necessary for displacement of a third methyl group
The width of the peptide-binding groove is crucial for catalysis, and the length separating the two edges is in between 7.two and seven.8 throughout all readily available structures of lively…