The overall RNAs extracted from the numerous CP and GFP expressed samples have been analyzed by agarose gel electrophoresis and blotted on to nylon membrane. The blot was hybridized by radioactive HcPro probes
C. Subcellular distribution in Nicotiana benthamiana epidermal cells of green fluorescent protein (GFP)-tagged PRSV HcPro and its mutant proteins M1 and M2, expressed transiently by agroinfiltration, as effectively as of…