Ed by a conserved internal Cys protease domain (CPD), which is activated upon the binding on the tiny molecule inositol polyphosphate (IP6). Affinity-tagged CPD is usually fused towards the C-terminus…
Aining procedure was adapted to assess the formation of HDL-like ApoE particles with TEM . Briefly, carbon-coated Formvar 400-mesh copper grids (AgarScientific, Stansted, UK) have been glow discharged before sample…
The polypeptides of globular proteins, the ribonuclease S-peptide (KETAAAKFERQHMDS and its Lys to Ala-substituted peptide AETAAAAFERQHMDS), the F-helix peptide of horse heart myoglobin (PLAQSH) or the made N-terminal oligo-Gly tag…
Nic actinomycete having a broad host variety, and causes serious industrial losses in the ornamental plant industry since it triggers malformations of shoots, known as leafy galls. In strain R.…
Endogenous AGTs do not acceptFig. 24 Self-labeling protein tags. a, b Both SNAP- and CLIP-tag derive from O6-methylguanine-DNA methyltransferase with C145 as the active web site. c The Halo-tag derives…
Rized SERCAs, discussed above, along with a much less characterized group of ATPases that have been described as secretory-pathway Ca2+ -ATPases (SPCAs; Shull, 2000; Figure 1; Table 1). The SPCAs…
Peptides is of recombinant origin, but the actual ligation step is still a chemical method and may be performed below a wide array of reactions to introduce a variety of…
Osporin A, which binds to CypD to inhibit mPTP, in mutant SOD1 mice, suggest that inhibition of mPTP could possibly be of advantage to ALS (Retain et al., 2001; Kirkinezos…
Qp-8::aqp-8::mCherry; Pfmo-4::NLS::GFP; B1-B4), CD1093 (Paqp-8::aqp-8::mCherry; Pfmo-4::NLS::gfp::LacZ; C1-C4) and CD1096 (Pfmo-4::mCherry ; Plin-48::GFP; D1-D4) visualized as merged green and red fluorescence channels either overlaid with (A1-A4,B3,B4,C1-C4,D1-D4) or without the need of…
C acyl tails and also the length from the carbon chains are believed to be important variables in determining the membrane fluidity (Fujimoto and Parmryd, 2017). Additionally, the lipid composition…