Thelization, and vascularization In vitro in vivo [171]Hyaluronic acid hydrogelGelMA hydrogelOral mucosa regenerationDecellularized AmnioM particlesGelMA AmnioM IgE Proteins web Particles [172] scaffold has been confirmed to become helpful in neovasculariza tion and mucosa repair In vitro and in vivo Significantly improve burn wound healing [175]Aloe vera gel Skin regenerationSkin regeneration (burn)Nondecellularized mem brane (powder) Decellularized hAmnioMNanofibrous FibroinIn vitroBilayer AmnioM/nanofibrous [174] fibroin scaffold represents an efficient all-natural construct with broad applicability to generate keratinocytes from Menstrual stem cells Decellularized hAmnioM In vitro in vivo The biocompatible scaffold could regenerate each soft and difficult tissue properly [192] Page 12 ofPOC polymerCleft palate repairTable 3 (continued)Goal Periodontal tissue regenera tion Decellularized hAmnioM In vitro Membrane status Study form Outcome RefEnhancement modalitiesAdditivesElkhenany et al. Stem Cell Analysis TherapyCombination with cellsDental pulp derived cellscell sheet that contained MSC [182] might be helpful for applica tion in periodontal tissue regeneration Wound healing with a [183] minimal scar in a fullthickness wound in rat back UAM provided a appropriate scaf fold for CSCs to create tis sue mimic the native cornea AMASCs accelerated the wound healing using a much less inflammatory response in a thirddegree burns rat model Higher drug entrapment was achieved by incubation of AmnioM for 3 h at 4C [193]TGF3 BMSCsSkin regenerationdehydrated AmnioM (hDAM) commercial ultrathin AmnioM In vitro and in vivoIn vitro in vivo(2022) 13:Corneal stromal cells (CSCs)Cornea regenerationASCsSkin regenerationDecellularized hAmnioMIn vitro and in vivo[184]Drug carrier Nanoreservoir Cornea regeneration hAmnioMCefazolinCornea regenerationhAmnioMIn vitro[179]MoxifloxacinIn vitroThick HAM entraps moxi [180] floxacin efficiently greater than thin HAM. 3 h incubation was enough for entrapment Clinical trial (Right after dermoid removal) In vitro and in vivo Rapid corneal reepithelization [194] and smooth healing Lysine amino acid could increase the crosslinking efficiency of AmnioM [195]Other additives Cornea regenerationTissue glueCornea regenerationIntact AmnioM Carbodiimide crosslinked AmnioM Decellularized hAmnioMAmino acidsCalcium and PhosphateBone regenerationIn vitro and in vivoThe mineralized AmnioM enhanced ASCs osteogenic differentiation in vitro and bone regeneration inside a calva rial bone defect in vivo[181]Page 13 ofElkhenany et al. Stem Cell Study Therapy(2022) 13:Page 14 ofto facilitate its applications, particularly within the corneal defects. Fibrin glue has been proposed by Szurman, Warga [176] as a bio-adhesive to stabilize the Amnio-M over the corneal surface. Nevertheless, in some situations, for instance Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) which need covering the complete cornea, the conjunctiva, at the same time as eyelid, securing a large sheet of Amnio-M was difficult. Shanbhag, Chodosh [177] proposed cyanoacrylate glue to fix Amnio-M in to the eyelid skin alongside applying a silicon ring to stabilize it over the cornea. An additional study on the treatment of recurrent retinal detachment making use of Amnio-M has shown that adding platelet-rich plasma (PRP) elevated the results rate of sealing the retinal hole [178]. Not too long ago, the drug reservoir properties with the AmnioM have already been investigated. They have been shown to Estrogen Receptor Proteins supplier effectively deliver bioactive molecules for instance c.