Product Name :
PKA kinase activity kit
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Store all components at 4°C, except active kinase at -80°C.
Description:
Non-radioactive protein kinase assay provides rapid and sensitive detection Safe and non-radioactive colorimetric measurement of kinase activity Flexible format, kinetic and end-point options available Fast results in Efficiency maximized with only 30 µl diluted sample needed per well The PKA Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols.{{9004-32-4} MedChemExpress|{9004-32-4} Purity & Documentation|{9004-32-4} In Vivo|{9004-32-4} supplier} The assay also offers a high signal to background ratio.{{1597403-47-8} web|{1597403-47-8} Protocol|{1597403-47-8} In Vivo|{1597403-47-8} supplier} PKA (Protein Kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP.PMID:31424841 Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits. PKA kinase activity kit Kit graph PKA kinase activity kit Kit graph
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| Alternative Name Protein kinase A, cAMP-dependent protein kinase | Application Colorimetric detection | Application Notes For the quantitative determination of PKA kinase activity in partially purified, purified, or crude enzyme preparations from any species. | Contents Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2 | Species Reactivity Species independent | UniProt ID P17612 (Cα subunit), P22694 (Cβ subunit), P22162 (Cγ subunit), P10644 (RIα subunit), P31321 (RIβ subunit), P13861 (RIIα subunit), P31323 (RIIβ subunit)