Th other mycotoxins. with other mycotoxins.Table two. Detection limit of test strip. Mass Concentration of AFB1 Common Answer ( /kg) 0 Chinese prickly ash Pepper Chili Cinnamon Aniseed three 6 12 25 50 0 Mass Concentration of OTA Common Option ( /kg) five ten 15 25 25 Spices- – – – — – – – -Note: “-” indicates adverse, “” indicates positive; AFB1 , aflatoxin B1 ; OTA, ochratoxin A.Foods 2021, 10, x2738 PEER Overview Foods 2021, 10, FOR7 7 of15 ofFigure two. (A) aflatoxin (AFB1) and (B) (B) ochratoxin A (OTA) test strip quantitative detection Figure two. (A) aflatoxin B1 B1 (AFB1) and ochratoxin A (OTA) test strip quantitative detection limit determination. limit determination.3.1.2. Precision limit of test strip. Table two. Detectionof Test Strips The precision of your test strip was reflected by the outcomes in the added Tasisulam MedChemExpress recovery test. It Mass Concentration of OTA Mass Concentration of AFB1 is usually noticed from Table three that the recovery price of AFB1 was 81.213.7 having a relative Option (g/kg) Common Resolution (g/kg) SpicesdeviationStandard less than 9.two . The recovery price of OTA was inside the range of standard (RSD) of three six 12 25 50 0 5 ten 25 25 82.218.6 with0an RSD of significantly less than eight.7 . In accordance with regulatory15 limits, a recovery Chinese rate among 80.020.0 with an RSD of much less than ten is thought of acceptable. From – – prickly and Table S1 ash Figure four, it could be noticed that the cross-reaction prices in the T1 line to AFB1 , AFB2 , AFG1 , and AFG2were one hundred.0 , 7.7 , four.2 , and less than 1.0 , respectively, and the Pepper – – T2 line had no cross-reactivity with these three structural analogs. This also means that if Chili – – you will find AFB1 structural analogues (AFB2 , AFG1 , and AFG2) inside the actual spice sample Cinnamon – – without the need of AFB1 , the test strip may perhaps show a optimistic outcome,- immediately after confirmation bythe HPLC but Aniseed – method, it can be ensured that there will probably be 1, aflatoxin B1; OTA, ochratoxin A. Note: “-” means damaging, “” suggests optimistic; AFBno false unfavorable results in the detection of AFB1 in spices, Evernic Acid Data Sheet thereby avoiding the possibility of missing the spice sample contaminated by AFB1 .Foods 2021, 10, x FOR PEER REVIEW8 8of 15 ofFigure 3. Test strip (A) T1 line and (B) T line specific test outcomes. Figure three. Test strip (A) T1 line and (B) T22line specific test outcomes. Table 3. Precision of test strips. three.1.2. Precision of Test StripsMycotoxins Add Level ( /kg) five.0 20.0 50.0 five.0 20.0 50.AFB1 OTAThe precisionPepperthe test strip wasChili of reflected by the results of your added recovery test. Cinnamon Aniseed RSD Recovery Recovery Recovery Recovery It can be observed from Tablethat the recovery rate of AFB1 was 81.213.7 having a relative RSD 3 RSD RSD RSD Rate Rate Rate Rate typical deviation (RSD) of less than 9.two .three.6The recovery price of OTA 111.0 five.6in the range was 83.2 two.eight two.6 81.2 six.six 6.1 105.three two.9 one hundred.four 2.eight three.3 five.9 102.8 3.9 4.three 110.0 2.7 9.2 103.4 five.two 83.6 six.8 6.two of 82.218.6 ith an 3.five RSD of 110.2 9.3than 8.7 . In accordance five.9 much less with regulatory limits, a 93.2 six.3 7.5 99.1 two.0 two.eight 113.7 three.7 four.1 92.9 six.9 six.3 110.four 2.four 2.six 101.2 four.six three.eight 95.eight 4.8 four.two 109.four 5.8 6.five 82.two 7.four eight.7 118.6 2.7 3.five recovery price among 80.020.0 with an three.0 RSD of 103.0 two.1 much less than 10 is regarded accepta113.0 five.1 5.3 109.three two.six 1.1 100.7 two.9 3.1 86.8 4.six 5.1 107.six two.9 4.six 92.1 four.five four.8 99.3 six.4 7.5 91.7 three.2 four.8 91.four three.four four.9 ble. From Table S1 and Figure 4, it might be observed.