DH-based PARP15 drug essential oil in comparison towards the FH-based important oil. The levels of creatinine, cholesterol, and triglycerides have been decreased (p 0.05) within the pre-treated rats by the critical oil batches, with non-significant variations identified amongst them because of the herbs dryings based oils. A notable enhance in the viability from the cells, and total antioxidant capacity (TAOxC) levels, collectively together with the reduction in malondialdehyde (MDA) levels were observed by the necessary oils obtained from all the batches as compared with all the AAP-treated cell-lines, HepG-2, HeLa, and MCF-7, that indicated the in vitro hepatoprotectiveMolecules 2021, 26, 5757. doi.org/10.3390/moleculesmdpi/journal/moleculesMolecules 2021, 26,2 ofeffects in the sage critical oils. Nonetheless, considerable improvements within the in vivo and in vitro hepatoprotective activities together with the 4WDH-based oil, as in comparison with all other essential oil-batches and silymarin common demonstrated the effective effects of the drying protocol for the herb for its medicinal purposes. Keyword phrases: Sage; Salvia officinalis; cytotoxicity; hepatoprotection; MDA; TAOxC; MCF-7; HeLA cells; HepG-2 cells1. Introduction Sage, salvia, or Salvia officinalis L., the plant belonging to the loved ones Lamiaceae, is often a perennial aromatic herb cultivated worldwide for its medicinal, culinary, and flavor properties. The sage, as a culinary and medicinal herb, is preferred worldwide and considered an P/Q-type calcium channel Biological Activity economically essential commodity for use within the apothecary and spices organization. Herbal shops regularly trade sage as well as other aromatic herbs wherein the herbs are dried at area temperature beneath shade to retain their aromatic contents. The herb is worldwide accessible and is employed in food preparations as well as as an necessary portion with the tea mixture with the Mediterranean nations [1]. The folk-medicinal and classic usage of sage is worldwide in nature, and the Latin name on the plant “Salvia” signifies curative, which can be indicative with the health-giving properties, and value of this plant [2]. The aerial components of the plant are utilised for the management of gout, rheumatism, diarrhea, and diabetes in the countries of Northern America, and Asia [3]. Sage can also be utilised for the treatment of gastrointestinal issues, for instance dyspepsia and ulcers, at the same time as heartburn, and upper respiratory complaints, including sore throat, and inflammations [1]. Sage also has noticeable valuable effects in age-related cognitive issues [4]. It truly is strongly reputed to induce calmness and boost memory in Alzheimer’s patients [5]. The plant leaves are frequently employed in the food industry as a result of their flavor and antioxidant, and antimicrobial properties [6]. The plant can also be part with the perfumery and cosmetic industries [2]. Chemical evaluation on the sage critical oil from distinctive places has been carried out, and detailed reports are available from various quarters. The main volatile constituents from the plant had been determined as getting -thujone, 1,8-cineole, and camphor, that are regarded as the chemotypic constituents from the S. officinalis vital oil [7]. The effects of seasonal variations [8], geographical areas variations in oil quality and yields [80], crucial oils’ extraction procedures [113], and the important oils obtained by means of unique drying approaches [14,15] have been reported as parts of the parameters affecting the plant essential oils’ yields and constituents. Significant variations amongst sage essential