Product Name :
Anti-Human IgG(CH2 Fragment specific), Mouse antibody(Biotin)
Applications:
IP,WB,ELISA
Reactivity :
Human IgG
Conjugate:
Biotin
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-Human IgG(CH2 Fragment specific), Mouse antibody(Biotin) is designed for detecting human IgG CH2 fragment specifically. Anti-Human IgG(CH2 Fragment specific), Mouse antibody(Biotin) is based on monoclonal, recombinant, mouse IgG1 Fc fused alpaca antibody to human IgG CH2 fragment coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG(CH2 Fragment specific), Mouse antibody(Biotin) reacts with human IgG CH2 fragment selectively, no reactivity with cynomolgus, rabbit, mouse, rat, goat IgG. | Immunogen: Human IgG | Host: Alpaca pacous | Isotype: Fab of Alpaca IgG1 fused to goat IgG Fc | Conjugate: Biotin | Specificity: Human IgG CH2 fragment | Cross-Reactivity:, No cross-reactivity with cynomolgus IgG, rabbit, mouse, rat, goat IgG | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.{{137281-23-3} site|{137281-23-3} Biological Activity|{137281-23-3} In Vivo|{137281-23-3} manufacturer} 1% trehalose, 0.01% proclin300 | Storage: Store at –20 °C(Avoid freeze / thaw cycles) | Background:In mammals, antibodies are classified into five main classes or isotypes – IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgG is the most abundant antibody in normal human serum, accounting for 70-85% of the total immunoglobulin pool. Human IgG consists of four human subclasses (IgG1, IgG2, IgG3 and IgG4), and each contains a different heavy chain. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda). The heavy chain is about 50 KD and the light chain is about 25 KD.{{75747-14-7} MedChemExpress|{75747-14-7} Purity & Documentation|{75747-14-7} Purity|{75747-14-7} supplier} The heavy chain chains consist of a variable domain, VH, and three constant domains CH1, CH2, and CH3.PMID:31424821 The common IgG is monomeric with a molecular weight of approximately 150 kD.
Description2 :
Anti-Human IgG(CH2 Fragment specific), Mouse antibody(Biotin) is designed for detecting human IgG CH2 fragment specifically. Anti-Human IgG(CH2 Fragment specific), Mouse antibody(Biotin) is based on monoclonal, recombinant, mouse IgG1 Fc fused alpaca antibody to human IgG CH2 fragment coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG(CH2 Fragment specific), Mouse antibody(Biotin) reacts with human IgG CH2 fragment selectively, no reactivity with cynomolgus, rabbit, mouse, rat, goat IgG.
Immunogen:
Human IgG
Host :
Alpaca pacous
Isotype:
Fab of Alpaca IgG1 fused to goat IgG Fc
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C(Avoid freeze / thaw cycles)
Function:
WB: 1:5000-1:20000ELISA： 1:5000-1:20000IP: 1-2ug/sampleDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.