Product Name :
Anti-HA tag, AlpSdAbs® VHH(iFluor488)
Applications:
WB,ICC/IF,ELISA,Flow Cyt
Reactivity :
HA tag
Conjugate:
iFluor488
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-HA tag, AlpSdAbs® VHH(iFluor488) is designed for detecting HA tag fusion proteins specifically. Anti-HA tag, AlpSdAbs® VHH(iFluor488) is based on monoclonal, recombinant, single domain antibody to HA tag coupled to iFluor488. Based on immunofluorescence and/or ELISA, Anti-HA tag, AlpSdAbs® VHH(iFluor488) detects the HA tag selectively, no reactivity with other proteins. | Immunogen: HA tag fused KLH | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c | Conjugate: iFluor488(Ex: 495nm, Em: 519nm) | Specificity: HA tag(YPYDVPDYA) | Cross-Reactivity: Highly selective for HA tag sequence | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PB(pH 7.{{196618-13-0} web|{196618-13-0} Technical Information|{196618-13-0} In Vitro|{196618-13-0} supplier} 5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol | Storage: Store at –20 °C, protect from light | Background:The HA tag is widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.{{2138299-34-8} site|{2138299-34-8} Biological Activity|{2138299-34-8} Data Sheet|{2138299-34-8} manufacturer} Because of its small size, HA tag is unlikely to affect the tagged protein’s biochemical properties. HA tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(
Description2 :
Anti-HA tag, AlpSdAbs® VHH(iFluor488) is designed for detecting HA tag fusion proteins specifically. Anti-HA tag, AlpSdAbs® VHH(iFluor488) is based on monoclonal, recombinant, single domain antibody to HA tag coupled to iFluor488.PMID:29489177 Based on immunofluorescence and/or ELISA, Anti-HA tag, AlpSdAbs® VHH(iFluor488) detects the HA tag selectively, no reactivity with other proteins.
Immunogen:
HA tag fused KLH
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C, protect from light
Function:
WB: 1:5000 -1:20000ELISA: 1:5000 -1:20000Flow Cyt: 1:200-1:2000ICC/IF: 1:200-1:2000Super-resolution microscopyDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.