NUCLEAR-ID® Red cell cycle kit (GFP-CERTIFIED®)

Description:
Convenient kit for studying cell cycle progression by various applications Complete kit provides DNA content information in live, permeabilized or fixed cells Stable and high purity far-red fluorescent dye Performance validated using a wide range of cell densities. Does not require RNase treatment. Monitors changes in cell cycle dynamics arising from drug treatment or other perturbations UV laser source is not required for excitation. No photobleaching effect. True multiplexed capability with additional probes and dyes Stringently manufactured to control and eliminate non-specific assay artifacts GFP and FITC compatible. Easy to use! Enzo Life Sciences’ GFP-CERTIFIED® NUCLEAR-ID® Red Cell Cycle Analysis Kit provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry. The kit is suitable for (1) determining the percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as to quantify cells in the sub-G1 phase, and (2) DNA studies in live, permeabilized and fixed cells for normal cell lines and cell lines exhibiting multiple ploidy levels. A control cell cycle perturbation agent, Nocodazole, is provided for monitoring changes in cell cycle dynamics. Potential applications for live-cell studies are in the determination of cellular DNA content and cell cycle distribution for the detection of variations in growth patterns, for monitoring apoptosis, and for evaluating tumor cell behavior and suppressor gene mechanisms. Relative costs of using Nuclear ID® Red DNA in comparison to competitor dye in various applications: (A) Imaging (visualization), (B) Nucleated Cell Gating (flow cytometry) and (C) Live Cell Cycle analysis using flow cytometry.{{479-61-8} MedChemExpress|{479-61-8} Biological Activity|{479-61-8} Data Sheet|{479-61-8} custom synthesis} Dilutions can vary depending on cell strain and cell concentration.Notes:Assumes staining of a 100µl staining volumeAssumes staining of a 500µl cell suspension volumeAssumes a staining of 500µl cell suspension volume NUCLEAR-ID® Red DNA Stain requires lower concentration than competitor’s dye to visualize dsDNA.HeLa cells were grown to ~60% confluency.{{107761-42-2} medchemexpress|{107761-42-2} Biological Activity|{107761-42-2} Description|{107761-42-2} custom synthesis} Cells were stained with NUCLEAR-ID® Red DNA Stain or a competitor’s dye at a final concentration of 2.5 or 5µM for 15 min at 37°C and gently washed post-staining. Cells were imaged at 15 min and 24h. Results show that 2.5µM NUCLEAR-ID® Red DNA Stain was required for visualization of the dsDNA, while 5.0µM was required for the competitor’s dye. At 24h, the competitor’s dye intensity and cell growth were dramatically reduced at the 5µM final concentration. At the same time point, 5µM of NUCLEAR-ID® Red DNA Stain did not affect cell growth or fluorescent intensity. The NUCLEAR-ID® Red DNA Stain shows lower cytotoxicity and requires lower concentration in live cell studies, resulting in lower costs. Figure 1: Three-dimensional reconstruction of the spatial relationship between the green fluorescent protein-expressing (GFP-expressing) mitochondria and the nucleus using a structured illumination method, as implemented with the ApoTome from Carl Zeiss, Inc. This imaging method enabled creation of optical sections through the nucleus using a conventional fluorescence microscope, for improved resolution along the optical axis. The optical sections were then used to create a 3-D reconstruction of the nucleus, enabling the GFP-expressing mitochondria to be displayed in their proper spatial context.PMID:30725978 Figure 2: Live cells treated with different drugs show inhibition of cell cycle progression at different phases. Relative costs of using Nuclear ID® Red DNA in comparison to competitor dye in various applications: (A) Imaging (visualization), (B) Nucleated Cell Gating (flow cytometry) and (C) Live Cell Cycle analysis using flow cytometry. Dilutions can vary depending on cell strain and cell concentration.Notes:Assumes staining of a 100µl staining volumeAssumes staining of a 500µl cell suspension volumeAssumes a staining of 500µl cell suspension volume NUCLEAR-ID® Red DNA Stain requires lower concentration than competitor’s dye to visualize dsDNA.HeLa cells were grown to ~60% confluency. Cells were stained with NUCLEAR-ID® Red DNA Stain or a competitor’s dye at a final concentration of 2.5 or 5µM for 15 min at 37°C and gently washed post-staining. Cells were imaged at 15 min and 24h. Results show that 2.5µM NUCLEAR-ID® Red DNA Stain was required for visualization of the dsDNA, while 5.0µM was required for the competitor’s dye. At 24h, the competitor’s dye intensity and cell growth were dramatically reduced at the 5µM final concentration. At the same time point, 5µM of NUCLEAR-ID® Red DNA Stain did not affect cell growth or fluorescent intensity. The NUCLEAR-ID® Red DNA Stain shows lower cytotoxicity and requires lower concentration in live cell studies, resulting in lower costs. Figure 1: Three-dimensional reconstruction of the spatial relationship between the green fluorescent protein-expressing (GFP-expressing) mitochondria and the nucleus using a structured illumination method, as implemented with the ApoTome from Carl Zeiss, Inc. This imaging method enabled creation of optical sections through the nucleus using a conventional fluorescence microscope, for improved resolution along the optical axis. The optical sections were then used to create a 3-D reconstruction of the nucleus, enabling the GFP-expressing mitochondria to be displayed in their proper spatial context. Figure 2: Live cells treated with different drugs show inhibition of cell cycle progression at different phases.