CTGCCGCGGCCTTCACCACCGCCGTCG-39 in the initial and 59-TATAGGATCCCCCGGGCCCGGGTTTTCTTCCACATCGCCGCAG-39 in the second reaction. The
CTGCCGCGGCCTTCACCACCGCCGTCG-39 within the very first and 59-TATAGGATCCCCCGGGCCCGGGTTTTCTTCCACATCGCCGCAG-39 inside the second reaction. The PCR product was digested with BamHI and sublconed into pHR-SIN-CSGW upstream of eGFP. After sequencing, a DEST cassette…