We isolated ALDHhigh and CD133-good TC71 cells by FACS (Determine 5E) and cultured 1000 cells/well of each population on extremely lower attachment plates supplemented with Mesencult to review their sphere forming capacity
Staining adjacent sections with CD99, a marker for ESFT cells (Determine 4B), verified that the ALDHpositive cells were indeed tumor cells colonies formed by the ALDHlow cells were greater than…