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acal silver solution for 1 hour at 37C. After further washing the cells twice with dH2O, they were examined by light microscopy using a Zeiss Axiovert 25 microscope. For staining…
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were harvested by centrifugation and washed once in 20 mM Tris-HCl, pH 8.0, before the cell pellet was frozen and stored until use. The frozen cell pellet was resuspended in…
calponin-3 in the S2 Schneider cell system. S2 cells were transfected with expression constructs encoding calponin-3 and Syk, PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19709857 is illustrated by a white box. LoxP-sites are purchase UNC0642…
NOS2/2 and treated iNOS2/2 displayed significantly lower inflammatory infiltrates compared to wt following 4 h reperfusion. 5 nNOS and Graft Reperfusion Effect of mouse donor genotype and BH4 treatment on…
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, we measured the pattern of MedChemExpress Luteolin 7-glucoside intracellular vesicles in macrophages exposed to CpG-DNA, which activates the TLR9 signaling cascade. In CpG-DNA-stimulated macrophages, YB-1 secretory vesicles were clearly…
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a microfluidic device at 37uC and 5% CO2. The imaging buffer consisted of 125 NaCl, 5.7 KCl, 2.5 CaCl2N2 H2O, 1.5 MgCl2, 10 HEPES, and 0.1% bovine serum albumin at…